genefish:bin Steven$ ./blastn -query /Volumes/web/whale/fish546/module7/GG2\ metagenome/GG2_AsmD_recon.fa -db /Users/Shared/data/blast/db/nt -out /Volumes/web/whale/fish546/module7/mod7_blastout_n_nt -max_target_seqs 1 -outfmt 6 -num_threads 8
genefish:bin Steven$ ./blastn -query /Volumes/web/whale/fish546/module7/GG2\ metagenome/GG2_AsmD_recon.fa -db /Users/Shared/data/blast/db/nt -out /Volumes/web/whale/fish546/module7/mod7_blastout_n_nt_blastn -max_target_seqs 1 -outfmt 6 -num_threads 8 -task blastn
280k lines
http://eagle.fish.washington.edu/whale/fish546/module7/mod7_blastout_n_nt_blastn
Taxonomic Rep in Galaxy
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./blastx -db /Volumes/web/whale/fish546/blast/db/uniprot_sprot -query /Volumes/web/whale/fish546/module7/GG2\ metagenome/GG2_AsmD_recon_v3.fa -out /Volumes/web/whale/fish546/module7/mod7_blastout_sp -max_target_seqs 1 -outfmt 6 -num_threads 16 -evalue 0.00000000001
JOINING partial (5khits) with SP in SQLshare
SELECT * FROM [sr320@washington.edu].[table_mod7_blastout_sp.txt]
LEFT JOIN [dhalperi@washington.edu].[SPID_GOnumber.txt]
ON [sr320@washington.edu].[table_mod7_blastout_sp.txt].Column3=[dhalperi@washington.edu].[SPID_GOnumber.txt].A0A000
--
SELECT * FROM [sr320@washington.edu].[mod7_fish546_sp]
LEFT JOIN [rkodner@washington.edu].[goslim_generic.obo]
ON [sr320@washington.edu].[mod7_fish546_sp].GO:0003824=[rkodner@washington.edu].[goslim_generic.obo].id
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SWISS-PROT Blast OUTPUT
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Getting more specific with metagenomic blastn
Filter blast to 1e-20
26k hits
